cd3e pe-cy5 antibody Search Results


anti cd3 phycoerythrin cyanin 5 pc5  (Miltenyi Biotec)
99
Miltenyi Biotec anti cd3 phycoerythrin cyanin 5 pc5
Anti Cd3 Phycoerythrin Cyanin 5 Pc5, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd3 phycoerythrin cyanin 5 pc5/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
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pe-cy5-conjugated anti-cd3e  (Thermo Fisher)
90
Thermo Fisher pe-cy5-conjugated anti-cd3e
A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells <t>(CD3e</t> + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.
Pe Cy5 Conjugated Anti Cd3e, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe-cy5-conjugated anti-cd3e/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
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anti-mouse cd4–fluorescein isothiocyanate (fitc)  (Thermo Fisher)
90
Thermo Fisher anti-mouse cd4–fluorescein isothiocyanate (fitc)
A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells <t>(CD3e</t> + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.
Anti Mouse Cd4–Fluorescein Isothiocyanate (Fitc), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-mouse cd4–fluorescein isothiocyanate (fitc)/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
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pe-cy5 hamster anti-mouse cd3e  (Becton Dickinson)
90
Becton Dickinson pe-cy5 hamster anti-mouse cd3e
A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells <t>(CD3e</t> + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.
Pe Cy5 Hamster Anti Mouse Cd3e, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe-cy5 hamster anti-mouse cd3e/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
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cd3e- pe cy5  (Thermo Fisher)
90
Thermo Fisher cd3e- pe cy5
A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells <t>(CD3e</t> + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.
Cd3e Pe Cy5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd3e- pe cy5/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
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cd3e  (Cytek Biosciences)
93
Cytek Biosciences cd3e
At 8dpi, pooled splenocytes (by genotype) from age matched, sex matched mice were sorted for live CD45+ cells and submitted for single cell RNA sequencing (scRNAseq) on the 10x genomic platform (A). Relative gene expression of Ptprc indicating immune cell in aggregated data set (both genotypes) (B). Relative gene expression to located immune cell clusters of myeloid Csfr1 , neutrophil Ly6g , B cell Cd19, and T cell <t>Cd3e</t> (C). Using Loupe software, each population was reclustered to group like cell subsets among the larger cell type and the proportions compared between genotypes. The frequency of each cluster among total cell population is listed on the graph. Myeloid cell cluster, based on Csf1r expression (D), Neutrophil clusters, based on Ly6g expression (E), and B cell cluster, based on Cd19 expression (F). T cells were further broken down into CD4 T cells (expression of Cd3e >0, Mt2 <10, Cd4 >0) (G) and CD8 T cells (expression of Cd3e >0, Mt2 <10, Cd8b1 >0) (H). tSNE plots highlighting Cd4 (G) and Cd8b1 (H) as well as breakdown of cluster for each T cell type in -WT and - 619WW cells. Corresponding quantification of proportion of either CD4 T cells or CD8 T cell subsets is next to tSNE plot.
Cd3e, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd3e/product/Cytek Biosciences
Average 93 stars, based on 1 article reviews
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pe cy5 anti mouse cd3e  (Thermo Fisher)
86
Thermo Fisher pe cy5 anti mouse cd3e
At 8dpi, pooled splenocytes (by genotype) from age matched, sex matched mice were sorted for live CD45+ cells and submitted for single cell RNA sequencing (scRNAseq) on the 10x genomic platform (A). Relative gene expression of Ptprc indicating immune cell in aggregated data set (both genotypes) (B). Relative gene expression to located immune cell clusters of myeloid Csfr1 , neutrophil Ly6g , B cell Cd19, and T cell <t>Cd3e</t> (C). Using Loupe software, each population was reclustered to group like cell subsets among the larger cell type and the proportions compared between genotypes. The frequency of each cluster among total cell population is listed on the graph. Myeloid cell cluster, based on Csf1r expression (D), Neutrophil clusters, based on Ly6g expression (E), and B cell cluster, based on Cd19 expression (F). T cells were further broken down into CD4 T cells (expression of Cd3e >0, Mt2 <10, Cd4 >0) (G) and CD8 T cells (expression of Cd3e >0, Mt2 <10, Cd8b1 >0) (H). tSNE plots highlighting Cd4 (G) and Cd8b1 (H) as well as breakdown of cluster for each T cell type in -WT and - 619WW cells. Corresponding quantification of proportion of either CD4 T cells or CD8 T cell subsets is next to tSNE plot.
Pe Cy5 Anti Mouse Cd3e, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe cy5 conjugated anti cd3e 145 2c11  (Thermo Fisher)
86
Thermo Fisher pe cy5 conjugated anti cd3e 145 2c11
A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells <t>(CD3e</t> + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.
Pe Cy5 Conjugated Anti Cd3e 145 2c11, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe cy5 conjugated anti cd3e 145 2c11/product/Thermo Fisher
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mouse anti-porcine cd3e-sprd (pe-cy5)  (SouthernBiotech)
90
SouthernBiotech mouse anti-porcine cd3e-sprd (pe-cy5)
A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells <t>(CD3e</t> + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.
Mouse Anti Porcine Cd3e Sprd (Pe Cy5), supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-porcine cd3e-sprd (pe-cy5)/product/SouthernBiotech
Average 90 stars, based on 1 article reviews
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pe-cy5.5-cd3e antibody  (Thermo Fisher)
90
Thermo Fisher pe-cy5.5-cd3e antibody
A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells <t>(CD3e</t> + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.
Pe Cy5.5 Cd3e Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe-cy5.5-cd3e antibody/product/Thermo Fisher
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cd3e-pecy5  (Thermo Fisher)
90
Thermo Fisher cd3e-pecy5
A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells <t>(CD3e</t> + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.
Cd3e Pecy5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd3e-pecy5/product/Thermo Fisher
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Image Search Results


A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells (CD3e + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.

Journal: bioRxiv

Article Title: Ym1 protein crystals promote type 2 immunity

doi: 10.1101/2023.07.03.547463

Figure Lengend Snippet: A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells (CD3e + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.

Article Snippet: BAL cells were stained with PE-conjugated anti-Siglec F (E50-2440) (BD Biosciences), PE-Cy5-conjugated anti-CD3e (145-2c11) (ThermoFisher Scientific), PE-Cy5-conjugated anti-CD19 (1D3) (Thermo Fisher Scientific), PE-Cy7-conjugated anti-CD11c (N418) (ThermoFisher Scientific), BD Horizon V450-conjugated anti-CD11b (M1/70) (BD Biosciences), Alexa Fluor 700-conjugated anti-Ly6G (1A8) (BD Biosciences) and APC-eFluor 780-conjugated anti-MHCII (M5/114.15.2) (Thermo Fisher Scientific).

Techniques: Adoptive Transfer Assay, Expressing

At 8dpi, pooled splenocytes (by genotype) from age matched, sex matched mice were sorted for live CD45+ cells and submitted for single cell RNA sequencing (scRNAseq) on the 10x genomic platform (A). Relative gene expression of Ptprc indicating immune cell in aggregated data set (both genotypes) (B). Relative gene expression to located immune cell clusters of myeloid Csfr1 , neutrophil Ly6g , B cell Cd19, and T cell Cd3e (C). Using Loupe software, each population was reclustered to group like cell subsets among the larger cell type and the proportions compared between genotypes. The frequency of each cluster among total cell population is listed on the graph. Myeloid cell cluster, based on Csf1r expression (D), Neutrophil clusters, based on Ly6g expression (E), and B cell cluster, based on Cd19 expression (F). T cells were further broken down into CD4 T cells (expression of Cd3e >0, Mt2 <10, Cd4 >0) (G) and CD8 T cells (expression of Cd3e >0, Mt2 <10, Cd8b1 >0) (H). tSNE plots highlighting Cd4 (G) and Cd8b1 (H) as well as breakdown of cluster for each T cell type in -WT and - 619WW cells. Corresponding quantification of proportion of either CD4 T cells or CD8 T cell subsets is next to tSNE plot.

Journal: bioRxiv

Article Title: Autoimmunity associated allele of tyrosine phosphatase gene PTPN22 enhances anti-viral immunity

doi: 10.1101/2023.06.28.546768

Figure Lengend Snippet: At 8dpi, pooled splenocytes (by genotype) from age matched, sex matched mice were sorted for live CD45+ cells and submitted for single cell RNA sequencing (scRNAseq) on the 10x genomic platform (A). Relative gene expression of Ptprc indicating immune cell in aggregated data set (both genotypes) (B). Relative gene expression to located immune cell clusters of myeloid Csfr1 , neutrophil Ly6g , B cell Cd19, and T cell Cd3e (C). Using Loupe software, each population was reclustered to group like cell subsets among the larger cell type and the proportions compared between genotypes. The frequency of each cluster among total cell population is listed on the graph. Myeloid cell cluster, based on Csf1r expression (D), Neutrophil clusters, based on Ly6g expression (E), and B cell cluster, based on Cd19 expression (F). T cells were further broken down into CD4 T cells (expression of Cd3e >0, Mt2 <10, Cd4 >0) (G) and CD8 T cells (expression of Cd3e >0, Mt2 <10, Cd8b1 >0) (H). tSNE plots highlighting Cd4 (G) and Cd8b1 (H) as well as breakdown of cluster for each T cell type in -WT and - 619WW cells. Corresponding quantification of proportion of either CD4 T cells or CD8 T cell subsets is next to tSNE plot.

Article Snippet: Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD3e (PE-Cy5/AF532, Tonbo Bioscience/Thermo Fischer, 1:200, clone 145-2C11), CD4 (PerCP/BV605, Tonbo Bioscience/Biolegend, 1:200, clone RM4-5), CD8α (APC-Cy7/APC-H7/APC, BD Biosciences, 1:200, clone 53-6.7), CD11c (PE-Cy5.5, Thermo Fisher, 1:100, clone N418), CD11b (PerCP-Cy5.5, Biolegend, 1:200, clone M1/70), F4/80 (Pacific Orange, Thermo Fisher, 1:100, clone BM8), IFNγ (AF647, Biolegend, 1:100, clone XMG1.2), TNFα (PE-Cy7, Beiolegend, 1:100, clone MP6-XT22), IL-2 (PE/BV421, Biolegend, 1:50, clone JES6-5H4), PDCA-1 (Pacific Blue, Biolegend, 1:200, clone 129C1), CD80 (BV421, 1:200, clone 16-10A1), CD86 (BV605, Biolegend, 1:200, clone GL1), PD-1 (PE-Cy7, Tonbo Biosciences, 1:200, clone J43.1), PD- L1 (PE/BV711, Tonbo Biosciences/Biolegend, 1:100, clone 10F.9G2), CD44 (AF700, Biolegend, 1:200, clone IM7), CD62L (FITC, Tonbo Biosciences, 1:100, MEL-14), Ly6C (BV785, Biolegend, 1:200, clone HK1.4), Ly6G (PE-eFlour610, Invitrogen, 1:200, clone IA8), CD206 (AF647, Biolegend, 1:100, clone CO68C2), CD209b (APC, Tonbo Biosciences, 1:200, clone 22D1), NK1.1 (FITC, Biolegend, 1:100, clone PK136), CD19 (BV711, Biolegend, 1:400, clone 6D5), B220 (APC-Cy5.5, Invitrogen, 1:200, clone RA3-6B2), MHC II I-Ab (FITC, Biolegend, 1:200, clone AF6-120.1), CXCR5 (BV605, Biolegend, 1:100, clone L138D7), Tbet (APC/AF647, Biolegend, 1:200), Foxp3 (PE, Invitrogen, 1:100, clone FJK-16s), LCMV-NP (self-conjugated to AF488 using Thermo Fisher AF488 labeling kit per manufacturer’s instructions, BioXcell, 1:50, clone VL-4).

Techniques: RNA Sequencing, Gene Expression, Software, Expressing

A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells (CD3e + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.

Journal: bioRxiv

Article Title: Ym1 protein crystals promote type 2 immunity

doi: 10.1101/2023.07.03.547463

Figure Lengend Snippet: A) Scheme representing the used adoptive transfer model. B-E) Proliferation and KN2 expression of OT-II T cells in the mLNs and lungs 4 and 7 days after i.t. administration of PBS, OVA alone, OVA plus Ym1 crystals or OVA plus soluble Ym1. (B) Representative histograms of the division profile of the adoptively transferred OT-II T cells (CD3e + CD4 + Vα2 + CD45.1.2 + ) in the mLNs on day 4. (C, D) The numbers of OT-II T cells and KN2+ OT-II T cells in the mLNs on day 4 (C) and day 7 (D). (E) The numbers of OT-II T cells and KN2+ OT-II T cells in the lungs on day 7. Data are pooled from 2 independent experiments with n = 10 mice per group. Data are shown as mean ± SEM. ns P ≥ 0.05; *P < 0.05; **P<0,01; ***P <0.001; ****P < 0.0001.

Article Snippet: For the analysis of innate immune responses, lung single-cell suspensions were stained for flow cytometry using FITC-conjugated anti-Ly-6C (AL-21) (BD Biosciences), PE-conjugated anti-Siglec-F (E50-2440) (BD Biosciences), PE-Cy5-conjugated anti-CD3e (145-2c11) (Thermo Fisher Scientific), PE-Cy5-conjugated anti-CD19 (1D3) (Thermo Fisher Scientific), PE-Cy7-conjugated anti-CD11c (N418) (Thermo Fisher Scientific), BD Horizon V450-conjugated anti-CD11b (M1/70) (BD Biosciences), Brilliant Violet 605-conjugated anti-CD45 (30-F11) (BD Biosciences), Alexa Fluor 647-conjugated anti-CD64 (X54-5/7.1) (BD Biosciences), Alexa Fluor 700– conjugated anti-Ly6G (1A8) (BD Biosciences) and APC-eFluor 780–conjugated anti-I-A/I-E (M5/114.15.2) (Thermo Fisher Scientific).

Techniques: Adoptive Transfer Assay, Expressing